Multiplexed detection of molecular biomarkers with phase-change nanoparticles

    Authors

    M. Su

    Comments

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    Abbreviated Journal Title

    Nanomedicine

    Keywords

    biomarker; detection; multiple; phase-change; nanoparticle; thermal; barcode; PROSTATE-CANCER; BIOLOGICAL DETECTION; GOLD NANOPARTICLES; ULTRASENSITIVE DETECTION; ELECTRICAL DETECTION; OVARIAN-CANCER; DNA; AMPLIFICATION; OPPORTUNITIES; MICROARRAYS; Biotechnology & Applied Microbiology; Nanoscience & Nanotechnology

    Abstract

    This review describes a new biosensing method based on nanoparticles of solid-to-liquid phase-change materials, in which a panel of metallic nanoparticles (metals and eutectic alloys) that have different compositions and melting temperatures are used as thermal reporters. Each type of nanoparticle will be conjugated to a ligand that can specifically bind to one type of molecular biomarker (protein or DNA) and then immobilized onto a substrate that is comodified with multiple ligands by forming sandwiched antibody antigen complexes or DNA double helices. After removing unbound nanoparticles by washing, the nature and concentration of the biomarkers are determined by detecting the melting temperature and fusion enthalpy of the nanoparticles using differential scanning calorimetry. Furthermore, an even larger panel of thermal barcodes can be formed by encapsulating selected phase-change nanoparticles inside non-melting shells, such as silica, where each microparticle will have a characteristic signature that can be determined from its thermal signatures.

    Journal Title

    Nanomedicine

    Volume

    8

    Issue/Number

    2

    Publication Date

    1-1-2013

    Document Type

    Review

    Language

    English

    First Page

    253

    Last Page

    263

    WOS Identifier

    WOS:000314791200019

    ISSN

    1743-5889

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