Thermally Addressed Immunosorbent Assay for Multiplexed Protein Detections Using Phase Change Nanoparticles

    Authors

    L. Y. Ma; C. M. Wang; Y. Hong; M. H. Zhang;M. Su

    Comments

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    Abbreviated Journal Title

    Anal. Chem.

    Keywords

    ULTRASENSITIVE DETECTION; BIOLOGICAL DETECTION; CANCER; NANOCRYSTALS; BISMUTH; DNA; Chemistry, Analytical

    Abstract

    Thermally addressed immunoassay is developed to detect multiple proteins using phase change nanoparticles as thermal barcodes. The solid to liquid phase changes of nanoparticles absorb heat energy and generate sharp melting peaks, which are used as thermal signatures to determine the existence and concentration of proteins. Multiple proteins can be detected by using different types of nanoparticles, in order to create a one-to-one correspondence between one type of nanoparticle and one type of protein. The fusion enthalpy that is proportional to the amount of phase change materials has been used to derive the amount of protein. The melting temperatures of nanoparticles are designed to be higher than 100 degrees C to avoid interference from species contained in the fluid. Thus, the use of thermal nanoparticles allows the detection of multiple low concentration proteins in a complex fluid such as cell lysate regardless of the color, salt concentration, and conductivity of the sample.

    Journal Title

    Analytical Chemistry

    Volume

    82

    Issue/Number

    4

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    1186

    Last Page

    1190

    WOS Identifier

    WOS:000274466100005

    ISSN

    0003-2700

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