Hydrolysis of DNA and its molecular components in the dry state

    Authors

    A. Marrone;J. Ballantyne

    Comments

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    Abbreviated Journal Title

    Forensic Sci. Int.-Genet.

    Keywords

    Hydrolysis of DNA; Dry state DNA; Depurination; PERFORMANCE LIQUID-CHROMATOGRAPHY; ACID-CATALYZED HYDROLYSIS; DEOXYRIBONUCLEIC-ACID; ABASIC SITE; CYTOSINE RESIDUES; DEAMINATION; MECHANISM; HEAT; PYRIMIDINES; DERIVATIVES; Genetics & Heredity; Medicine, Legal

    Abstract

    The biochemistry of dry state DNA is of interest to the field of forensic biology. The precise chemical nature of the hydrolytic degradation products of the DNA molecule in the dry state has not been previously investigated in detail. In this study we found that the mechanistic chemistry of DNA hydrolysis appears to be the same for the hydrated and dry states. The thermodynamic parameters are also similar in both states and the activation energies for base hydrolysis are indistinguishable. The principal difference between the two states is the rate at which hydrolytic degradation occurs. The duplex configuration of dry state DNA offers much more protection for the molecule than is offered in single strand and nucleotide species. Single strand breaks of dry state duplex DNA occur with a half life of 24 +/- 2 days at 65 degrees C and appear to occur in a mechanistic manner. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

    Journal Title

    Forensic Science International-Genetics

    Volume

    4

    Issue/Number

    3

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    168

    Last Page

    177

    WOS Identifier

    WOS:000276456200004

    ISSN

    1872-4973

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