A phenotypic culture system for the molecular analysis of CNS myelination in the spinal cord

    Authors

    H. Davis; M. Gonzalez; M. Stancescu; R. Love; J. J. Hickman;S. Lambert

    Comments

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    Abbreviated Journal Title

    Biomaterials

    Keywords

    Myelination; Spinal cord; Motoneurons; Oligonucleotide; Surface; chemistry; Oligodendrocyte precursor; EMBRYONIC RAT MOTONEURONS; SERUM-FREE MEDIUM; IN-VITRO MODEL; ORGANOSILANE SURFACE; MONOCLONAL-ANTIBODY; DEFINED SYSTEM; MOTOR-NEURONS; PROTEIN CASPR; OLIGODENDROCYTES; GROWTH; Engineering, Biomedical; Materials Science, Biomaterials

    Abstract

    Studies of central nervous system myelination lack defined in vitro models which would effectively dissect molecular mechanisms of myelination that contain cells of the correct phenotype. Here we describe a co-culture of purified motoneurons and oligodendrocyte progenitor cells, isolated from rat embryonic spinal cord using a combination of immunopanning techniques. This model illustrates differentiation of oligodendrocyte progenitors into fully functional mature oligodendrocytes that myelinate axons. It also illustrates a contribution of axons to the rate of oligodendrocyte maturation and myelin gene expression. The defined conditions used allow molecular analysis of distinct stages of myelination and precise manipulation of inductive cues affecting axonal-oligodendrocyte interactions. This phenotypic in vitro myelination model can provide valuable insight into our understanding of demyelinating disorders, such as multiple sclerosis and traumatic diseases such as spinal cord injury where demyelination represents a contributing factor to the pathology of the disorder. (C) 2014 Elsevier Ltd. All rights reserved.

    Journal Title

    Biomaterials

    Volume

    35

    Issue/Number

    31

    Publication Date

    1-1-2014

    Document Type

    Article

    Language

    English

    First Page

    8840

    Last Page

    8845

    WOS Identifier

    WOS:000340984600009

    ISSN

    0142-9612

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