Enzyme-assisted target recycling (EATR) for nucleic acid detection

    Authors

    Y. V. Gerasimova;D. M. Kolpashchikov

    Comments

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    Abbreviated Journal Title

    Chem. Soc. Rev.

    Keywords

    ULTRASENSITIVE ELECTROCHEMICAL DETECTION; ROLLING CIRCLE AMPLIFICATION; CYCLING PROBE TECHNOLOGY; HYBRIDIZATION CHAIN-REACTION; SEQUENCE-SPECIFIC DETECTION; RESONANCE ENERGY-TRANSFER; DUPLEX-SPECIFIC; NUCLEASE; LABEL-FREE DETECTION; OF-CARE DIAGNOSTICS; ENDONUCLEASE SIGNAL; AMPLIFICATION; Chemistry, Multidisciplinary

    Abstract

    Fast, reliable and sensitive methods for nucleic acid detection are of growing practical interest with respect to molecular diagnostics of cancer, infectious and genetic diseases. Currently, PCR-based and other target amplification strategies are most extensively used in practice. At the same time, such assays have limitations that can be overcome by alternative approaches. There is a recent explosion in the design of methods that amplify the signal produced by a nucleic acid target, without changing its copy number. This review aims at systematization and critical analysis of the enzyme-assisted target recycling (EATR) signal amplification technique. The approach uses nucleases to recognize and cleave the probe-target complex. Cleavage reactions produce a detectable signal. The advantages of such techniques are potentially low sensitivity to contamination and lack of the requirement of a thermal cycler. Nucleases used for EATR include sequence-dependent restriction or nicking endonucleases or sequence independent exonuclease III, lambda exonuclease, RNase H, RNase HII, AP endonuclease, duplex-specific nuclease, DNase I, or T7 exonuclease. EATR-based assays are potentially useful for point-of-care diagnostics, single nucleotide polymorphisms genotyping and microRNA analysis. Specificity, limit of detection and the potential impact of EATR strategies on molecular diagnostics are discussed.

    Journal Title

    Chemical Society Reviews

    Volume

    43

    Issue/Number

    17

    Publication Date

    1-1-2014

    Document Type

    Review

    Language

    English

    First Page

    6405

    Last Page

    6438

    WOS Identifier

    WOS:000340705900011

    ISSN

    0306-0012

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