Abbreviated Journal Title
Clin. Vaccine Immunol.
Keywords
ACQUIRING SURFACE-PROTEINS; BORRELIA-BURGDORFERI; IR6 PEPTIDE; SERODIAGNOSIS; FIBRONECTIN; DIAGNOSIS; ASSAY; VLSE; OSPC; GENE; Immunology; Infectious Diseases; Microbiology
Abstract
A serology-based tiered approach has, to date, provided the most effective means of laboratory confirmation of clinically suspected cases of Lyme disease, but it lacks sensitivity in the early stages of disease and is often dependent on subjectively scored immunoblots. We recently demonstrated the use of immuno-PCR (iPCR) for detecting Borrelia burgdorferi antibodies in patient serum samples that were positive for Lyme disease. To better understand the performance of the Lyme disease iPCR assay, the repeatability and variability of the background of the assay across samples from a healthy population (n = 36) were analyzed. Both of these parameters were found to have coefficients of variation of < 3%. Using eight antigen-specific iPCR assays and positive call thresholds established for each assay, iPCR IgM and/or IgG diagnosis from Lyme disease patient serum samples (n = 12) demonstrated a strong correlation with that of 2-tier testing. Furthermore, a simplified iPCR approach using a single hybrid antigen and detecting only IgG antibodies confirmed the 2-tier diagnosis in the Lyme disease patient serum samples (n = 12). Validation of the hybrid antigen IgG iPCR assay using a blinded panel of Lyme disease and non-Lyme disease patient serum samples (n = 92) resulted in a sensitivity of 69% (95% confidence interval [CI], 50% to 84%), compared to that of the 2-tier analysis at 59% (95% CI, 41% to 76%), and a specificity of 98% (95% CI, 91% to 100%) compared to that of the 2-tier analysis at 97% (95% CI, 88% to 100%). A single-tier hybrid antigen iPCR assay has the potential to be an improved method for detecting host-generated antibodies against B. burgdorferi.
Journal Title
Clinical and Vaccine Immunology
Volume
21
Issue/Number
8
Publication Date
1-1-2014
Document Type
Article
Language
English
First Page
1094
Last Page
1105
WOS Identifier
ISSN
1556-6811
Recommended Citation
Halpern, Micah D.; Molins, Claudia R.; Schriefer, Martin; and Jewett, Mollie W., "Simple Objective Detection of Human Lyme Disease Infection Using Immuno-PCR and a Single Recombinant Hybrid Antigen" (2014). Faculty Bibliography 2010s. 5408.
https://stars.library.ucf.edu/facultybib2010/5408
Comments
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