Title

Redox-active nanoceria depolarize mitochondrial membrane of human colon cancer cells

Authors

Authors

S. K. Jana; P. Banerjee; S. Das; S. Seal;K. Chaudhury

Comments

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Abbreviated Journal Title

J. Nanopart. Res.

Keywords

Nanoceria; Colon cancer; Reactive oxygen species; Mitochondrial membrane; potential; Lipid peroxidation; Nanomedicine; CERIUM OXIDE NANOPARTICLES; Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials; Science, Multidisciplinary

Abstract

Nanotherapeutics is emerging as a promising option to the various limitations and side effects associated with conventional chemotherapy. The present study investigates the cytotoxic effect of redox-active cerium oxide nanoparticles (nanoceria) on human colorectal adenocarcinoma-derived cell line (HCT 15). Exposure of these cells to nanoceria for 24 h with concentration ranging between 10 and 100 mu M resulted in a significant reduction of cell viability in a dose-dependent manner. Further, at a concentration of 10 A mu M, nanoceria exhibited time-dependent cytotoxic effect when exposed to the cells for 24, 48, and 72 h. Upon treatment of the cells with nanoceria, reactive oxygen species (ROS) and lipid peroxidation which are indicators of oxidative stress and cytotoxicity increased significantly, in a dose-dependent manner. Nanoceria was also found to depolarize the mitochondrial membrane, thereby collapsing the membrane potential and leading to initiation of apoptosis. Scanning electron microscopic study of nanoceria-treated HCT 15 cells showed morphological changes and loss of filopodia and lamellipodia, indicating arrest of metastatic spread. Summarizing, when cultured HCT 15 cells are exposed to nanoceria, a dose-dependent cytotoxic effect mediated by ROS generation is observed.

Journal Title

Journal of Nanoparticle Research

Volume

16

Issue/Number

6

Publication Date

1-1-2014

Document Type

Article

Language

English

First Page

9

WOS Identifier

WOS:000337344500001

ISSN

1388-0764

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