Title

Novel Hydrophilic Bis(1,2,3-triazolyl)fluorenyl Probe for In vitro Zinc Ion Sensing

Authors

Authors

D. M. Nguyen; X. H. Wang; H. Y. Ahn; L. Rodriguez; M. V. Bondar;K. D. Belfield

Comments

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Abbreviated Journal Title

ACS Appl. Mater. Interfaces

Keywords

1, 2, 3-triazolyl-fluorene; two-photon microscopy bioimaging; fluorescent; probe; zinc ion sensing; metal sensing; binding constant; ABSORBING FLUORENE DERIVATIVES; ONE-PHOTON; PHOTOCHEMICAL PROPERTIES; 2-PHOTON MICROSCOPY; FLUORESCENT SENSOR; CLICK CHEMISTRY; CROSS-SECTION; DESIGN; PHOTOSTABILITY; EXCITATION; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary

Abstract

A hydrophillic bis(1,2,3-triazoly1)fluorene derivative was synthesized as a multi-photon absorbing zinc-ion sensing fluorescent probe. The fluorescent response was approximately five fold greater in presence of Zn(2+) resulting in a large binding constant (1 x 10(9)) for a 1/2 ligand to zinc complex. A four fold increase in the two-photon absorption cross section was achieved upon binding Zn(2+). In vitro two photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn2(+) into HeLa cells and reversible Zn(2+) binding demonstrating the potential of this probe for zinc ion sensing.

Journal Title

Acs Applied Materials & Interfaces

Volume

2

Issue/Number

11

Publication Date

1-1-2010

Document Type

Letter

Language

English

First Page

2978

Last Page

2981

WOS Identifier

WOS:000284454400004

ISSN

1944-8244

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