A multiplexed chip-based assay system for investigating the functional development of human skeletal myotubes in vitro

    Authors

    A. S. T. Smith; C. J. Long; K. Pirozzi; S. Najjar; C. McAleer; H. H. Vandenburgh;J. J. Hickman

    Comments

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    Abbreviated Journal Title

    J. Biotechnol.

    Keywords

    Human; Skeletal muscle; Cantilevers; Functional assay; Body-on-a-chip; CONTRACTILE PROPERTIES; MUSCLE; CELLS; EXCITABILITY; INTEGRATION; CULTURE; TISSUES; 3D; Biotechnology & Applied Microbiology

    Abstract

    This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal myotubes. Data is presented demonstrating the survival and differentiation of human myotubes on microscale silicon cantilevers in a defined, serum-free system. These cultures can be stimulated electrically and the resulting contraction quantified using modified atomic force microscopy technology. This system provides a higher degree of sensitivity for investigating contractile waveforms than video-based analysis, and represents the first system capable of measuring the contractile activity of individual human muscle myotubes in a reliable, high-throughput and non-invasive manner. The development of such a technique is critical for the advancement of body-on-a-chip platforms toward application in pre-clinical drug development screens. (C) 2014 Elsevier B.V. All rights reserved.

    Journal Title

    Journal of Biotechnology

    Volume

    185

    Publication Date

    1-1-2014

    Document Type

    Article

    Language

    English

    First Page

    15

    Last Page

    18

    WOS Identifier

    WOS:000341046100003

    ISSN

    0168-1656

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