Title
A multiplexed chip-based assay system for investigating the functional development of human skeletal myotubes in vitro
Abbreviated Journal Title
J. Biotechnol.
Keywords
Human; Skeletal muscle; Cantilevers; Functional assay; Body-on-a-chip; CONTRACTILE PROPERTIES; MUSCLE; CELLS; EXCITABILITY; INTEGRATION; CULTURE; TISSUES; 3D; Biotechnology & Applied Microbiology
Abstract
This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal myotubes. Data is presented demonstrating the survival and differentiation of human myotubes on microscale silicon cantilevers in a defined, serum-free system. These cultures can be stimulated electrically and the resulting contraction quantified using modified atomic force microscopy technology. This system provides a higher degree of sensitivity for investigating contractile waveforms than video-based analysis, and represents the first system capable of measuring the contractile activity of individual human muscle myotubes in a reliable, high-throughput and non-invasive manner. The development of such a technique is critical for the advancement of body-on-a-chip platforms toward application in pre-clinical drug development screens. (C) 2014 Elsevier B.V. All rights reserved.
Journal Title
Journal of Biotechnology
Volume
185
Publication Date
1-1-2014
Document Type
Article
Language
English
First Page
15
Last Page
18
WOS Identifier
ISSN
0168-1656
Recommended Citation
"A multiplexed chip-based assay system for investigating the functional development of human skeletal myotubes in vitro" (2014). Faculty Bibliography 2010s. 6098.
https://stars.library.ucf.edu/facultybib2010/6098
Comments
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