Analysis of Tumor Mitotic Rate in Thin Metastatic Melanomas Compared With Thin Melanomas Without Metastasis Using Both the Hematoxylin and Eosin and Anti-phosphohistone 3 IHC Stain

    Authors

    S. Thareja; J. S. Zager; D. Sadhwani; S. Thareja; R. Chen; S. Marzban; D. M. Jukic; L. F. Glass;J. Messina

    Comments

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    Abbreviated Journal Title

    Am. J. Dermatopathol.

    Keywords

    AMERICAN JOINT COMMITTEE; LYMPH-NODE BIOPSY; PRIMARY CUTANEOUS MELANOMA; MALIGNANT-MELANOMA; SENTINEL; POSITIVITY; PREDICTORS; DISEASE; Dermatology

    Abstract

    Studies have suggested that elevated tumor mitotic rate (MR) is linked to overall survival in thin melanoma. Recently, promising data regarding anti-phosphohistone 3 (pHH3) immunohistochemistry and its ability to aid in calculation of MR have emerged. The authors retrospectively analyzed original biopsies from 13 thin melanomas with positive sentinel node (SN) status and 16 thin melanomas with negative SN status. Both anti-pHH3 immunohistochemistry and the hematoxylin and eosin (H&E) stain were used to evaluate MR by 2 dermatopathologists blinded to SN status using the hot spot method. Intraclass coefficient values were attained to measure interobserver concordance and reliability of the pHH3 stain. By generating a receiver operating characteristic curve and analyzing the overall area under the curve, pHH3 was found to have good interobserver reliability. The relationship between MR and SN involvement was also evaluated, but this correlation was not statistically significant.

    Journal Title

    American Journal of Dermatopathology

    Volume

    36

    Issue/Number

    1

    Publication Date

    1-1-2014

    Document Type

    Article

    Language

    English

    First Page

    64

    Last Page

    67

    WOS Identifier

    WOS:000331561000009

    ISSN

    0193-1091

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