Label free detection of the breast cancer biomarker CM15.3 using ZnO nanorods coated quartz crystal microbalance

    Authors

    X. H. Wang; H. Y. Yu; D. D. Lu; J. Zhang;W. W. Deng

    Comments

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    Abbreviated Journal Title

    Sens. Actuator B-Chem.

    Keywords

    ZnO nanorods; CA15.3; Antibody; Biosensor; Quartz crystal microbalance; ELECTRON-TRANSFER; IMMOBILIZATION; IMMUNOSENSOR; NANOTUBES; BIOSENSOR; PROTEINS; ARRAYS; ELECTROCHEMISTRY; NANOPARTICLES; TYROSINASE; Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation

    Abstract

    The sensing properties of ZnO nanorods which were immobilized with CA15.3 antibody (anti-CA15.3) for CA15.3 detection were studied by using quartz crystal microbalance (QCM) sensors in this work. ZnO nanorods were synthesized at 90 degrees C by wet chemical route which was used to grow the ZnO nanorods directly on the QCM. The sensors were constructed by binding anti-CA15.3 on ZnO nanorods surface using 3-aminopropyltrietoxy silane (APTES) and glutaraldehyde and were used for CA15.3 detection. The morphology of ZnO nanorods was examined by scanning electron microscopy (SEM). The SEM results indicated the grown ZnO nanorods were well-aligned and the diameter was around 100 nm. These QCM sensors showed high sensitivity (25.34 +/- 0.67 Hz/scale (1 U/mL)) and good linearity (0.99) in the concentration range of CA15.3 from 0.5 to 26 U/mL by the experiment. The response time of these sensors were observed less than 10 s with good reproducibility and stability. All the results indicated that one-dimensional ZnO nanostructure is a good material for biosensor application. (C) 2014 Elsevier B.V. All rights reserved.

    Journal Title

    Sensors and Actuators B-Chemical

    Volume

    195

    Publication Date

    1-1-2014

    Document Type

    Article

    Language

    English

    First Page

    630

    Last Page

    634

    WOS Identifier

    WOS:000332417600083

    ISSN

    0925-4005

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