Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general

    Authors

    S. Y. Zhang; A. Garcia-D'Angeli; J. P. Brennan;Q. Huo

    Comments

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    Abbreviated Journal Title

    Analyst

    Keywords

    SURFACE-PLASMON RESONANCE; HORSERADISH-PEROXIDASE; CANCER BIOMARKERS; PROSTATE-CANCER; ANTIBODY-AFFINITY; PROTEIN-DETECTION; CHEMILUMINESCENCE; SENSITIVITY; NANOTECHNOLOGY; NANOPARTICLES; Chemistry, Analytical

    Abstract

    The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information presented in this article may be applied to other types of biomolecular assays and analytical techniques. A clear understanding of what affects the detection limit can help researchers to evaluate different bio-analytical techniques properly, and to design better strategies to optimize and achieve the best analytical performance.

    Journal Title

    Analyst

    Volume

    139

    Issue/Number

    2

    Publication Date

    1-1-2014

    Document Type

    Article

    Language

    English

    First Page

    439

    Last Page

    445

    WOS Identifier

    WOS:000328642700012

    ISSN

    0003-2654

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