Different Interaction Modes of Biomolecules with Citrate-Capped Gold Nanoparticles

    Authors

    S. Y. Zhang; Y. Moustafa;Q. Huo

    Comments

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    Abbreviated Journal Title

    ACS Appl. Mater. Interfaces

    Keywords

    gold nanoparticle; protein corona; protein-nanoparticle interaction; dynamic light scattering; polysaccharides; hyaluronan; BOVINE SERUM-ALBUMIN; HYALURONIC-ACID; PROSTATE-CANCER; PARTICLE-SIZE; LIGHT-SCATTERING; BLADDER-CANCER; HUMAN BLOOD; HUMAN-IGG; BINDING; PLASMA; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary

    Abstract

    In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, zeta-potential analysis, UVvis absorption spectroscopy, and transmission electron microscopy. The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan. BSA, IgG, and IgM are high abundance proteins in blood. Hyaluronan is a major component of the extracellular matrix. An abnormal level of hyaluronan in blood is associated with a number of medical conditions including rheumatoid arthritis and malignancy. Five different interaction modes were observed from these molecules. While BSA and IgM interact with the gold nanoparticles by forming electrostatic interactions with the citrate ligands, IgG and hyaluronan adsorb to the nanoparticle metal core by displacing the citrate ligands. BSA, rabbit IgG, and hyaluronan formed a stable monolayer on the nanoparticle surface. Human IgG and IgM caused nanoparticle cluster formation upon interacting with the gold nanoparticles. For the first time, we discovered that hyaluronan, a highly negatively charged polyglycosaminoglycan, exhibits an exceptionally strong affinity toward the citrategold nanoparticles. It can effectively compete with IgG to adsorb to the gold nanoparticles. This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.

    Journal Title

    Acs Applied Materials & Interfaces

    Volume

    6

    Issue/Number

    23

    Publication Date

    1-1-2014

    Document Type

    Article

    Language

    English

    First Page

    21184

    Last Page

    21192

    WOS Identifier

    WOS:000346326600074

    ISSN

    1944-8244

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