Title
Leukocyte IGF-1 Receptor Expression during Muscle Recovery
Abbreviated Journal Title
Med. Sci. Sports Exerc.
Keywords
MUSCLE REPAIR; IMMUNE FUNCTION; COLD WATER IMMERSION; CRYOTHERAPY; RESISTANCE EXERCISE; INFLAMMATION; GROWTH-FACTOR-I; HEAVY-RESISTANCE EXERCISE; SKELETAL-MUSCLE; INJURY; MACROPHAGES; MONOCYTES; REGENERATION; LYMPHOCYTE; NEUTROPHIL; RESPONSES; Sport Sciences
Abstract
Introduction The insulin-like growth factor 1 (IGF-1) system plays a central role in anabolic cellular processes. Recently, a regulatory role of IGF-1 in the immune response for muscle repair has been suggested, but how it modulates the inflammatory process is largely unknown. We evaluated changes in leukocyte expression of IGF-1 receptors (IGF-1R) during recovery from resistance exercise to determine whether changes in the potential for IGF-1 interactions with leukocytes may mediate the role of IGF-1 in muscle repair. Methods Twenty resistance-trained men (18-35 yr) performed resistance exercise followed by cold water immersion (CWI) or control treatment (CON) on three consecutive days. Blood was sampled at baseline (PRE), immediately (IP), 30 min (30P), 24 h (24H), and 48 h after (48H) exercise. Circulating IGF-1 was assayed, and IGF-1 receptor expression (CD221) on gated circulating leukocytes (monocytes, granulocytes, and lymphocytes) was measured by flow cytometry. Time and treatment effects were analyzed with ANCOVA. Results Circulating IGF-1 significantly increased from PRE to IP as a result of resistance exercise, but no differences between CON and CWI were observed. Mean fluorescence intensity of CD221 on monocytes and granulocytes and percent of CD221+ granulocytes significantly increased at 30P (P < 0.000) and returned to preexercise levels by 24H. No treatment effects on monocytes or granulocytes were observed. On lymphocytes, mean fluorescence intensity of CD221+ significantly increased from PRE to 30P in CWI. Conclusions Changes in IGF-1 and its receptor on monocytes and granulocytes seem to be part of the mechanism that facilitates recovery from resistance exercise during earlier stages of muscle recovery. In addition, CWI seems to alter IGF-mediated responses on slower-acting lymphocytes, suggesting that its effects may be seen in later stages of muscle repair.
Journal Title
Medicine and Science in Sports and Exercise
Volume
47
Issue/Number
1
Publication Date
1-1-2015
Document Type
Article
Language
English
First Page
92
Last Page
99
WOS Identifier
ISSN
0195-9131
Recommended Citation
"Leukocyte IGF-1 Receptor Expression during Muscle Recovery" (2015). Faculty Bibliography 2010s. 6535.
https://stars.library.ucf.edu/facultybib2010/6535
Comments
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