A photosensitive surface capable of inducing electrophysiological changes in NG108-15 neurons

    Authors

    F. Huang; J. Bladon; R. C. Lagoy; P. N. Shorrock; M. Hronik-Tupaj; C. A. Zoto; R. E. Connors; W. G. McGimpsey; P. Molnar; S. Lambert; A. R. Rittenhouse;C. R. Lambert

    Comments

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    Abbreviated Journal Title

    Acta Biomater.

    Keywords

    Current clamp; Neuronal stimulation; Retinal prosthetic; Self-assembled; monolayer (SAM); Voltage clamp; SELF-ASSEMBLED MONOLAYERS; RETINAL PROSTHESES; ELECTROCHEMICAL; CHARACTERIZATION; ELECTRON-TRANSFER; DYE; INTERFACE; CELLS; WETTABILITY; AU(111); SAFETY; Engineering, Biomedical; Materials Science, Biomaterials

    Abstract

    Retinal prostheses promise to be a viable therapy for many forms of blindness. Direct stimulation of neurons using an organic light-sensitive, self-assembled monolayer surface offers a simple alternative to conventional semiconductor technology. For this purpose we have derivatized an indium tin oxide (ITO) substrate with the photosensitive dye, NK5962, using 3-(aminopropyl)trimethoxysilane (APTMS) as cross-linker. The surface was characterized through contact angle goniometry, electrochemical impedance spectroscopy, grazing angle infrared and ultraviolet visible spectrophotometry. NG108-15 neurons were grown on the ITO-APTMS-NK5962 surface and neural responses from electrical stimulation vs. photostimulation through the ITO-APTMS-NK5962 surface were measured using patch clamp electrophysiology. Under these conditions, photostimulation of depolarized cells caused an approximate 2-fold increase in voltage-gated sodium (Na+) current amplitude at a membrane potential of -30 mV. Our results demonstrate the feasibility of stimulating neurons, grown on light-sensitive surfaces, with light impulses, which ultimately may facilitate the fabrication of a simple, passive retinal prosthetic. (C) 2014 Published by Elsevier Ltd. on behalf of Acta Materialia Inc.

    Journal Title

    Acta Biomaterialia

    Volume

    12

    Publication Date

    1-1-2015

    Document Type

    Article

    Language

    English

    First Page

    42

    Last Page

    50

    WOS Identifier

    WOS:000348686100005

    ISSN

    1742-7061

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