Optofluidic ultrahigh-throughput detection of fluorescent drops

    Authors

    M. Kim; M. Pan; Y. Gai; S. Pang; C. Han; C. Yang;S. K. Y. Tang

    Comments

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    Abbreviated Journal Title

    Lab Chip

    Keywords

    IMAGE SENSORS; MICROFLUIDICS; DROPLETS; SINGLE; ARRAY; EMULSIONS; EVOLUTION; SYSTEMS; CHIP; Biochemical Research Methods; Chemistry, Multidisciplinary; Nanoscience; & Nanotechnology

    Abstract

    This paper describes an optofluidic droplet interrogation device capable of counting fluorescent drops at a throughput of 254000 drops per second. To our knowledge, this rate is the highest interrogation rate published thus far. Our device consists of 16 parallel microfluidic channels bonded directly to a filter-coated two-dimensional Complementary Metal-Oxide-Semiconductor (CMOS) sensor array. Fluorescence signals emitted from the drops are collected by the sensor that forms the bottom of the channel. The proximity of the drops to the sensor facilitates efficient collection of fluorescence emission from the drops, and overcomes the trade-off between light collection efficiency and field of view in conventional microscopy. The interrogation rate of our device is currently limited by the acquisition speed of CMOS sensor, and is expected to increase further as high-speed sensors become increasingly available.

    Journal Title

    Lab on a Chip

    Volume

    15

    Issue/Number

    6

    Publication Date

    1-1-2015

    Document Type

    Article

    Language

    English

    First Page

    1417

    Last Page

    1423

    WOS Identifier

    WOS:000350543500003

    ISSN

    1473-0197

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