Title

Optofluidic ultrahigh-throughput detection of fluorescent drops

Authors

Authors

M. Kim; M. Pan; Y. Gai; S. Pang; C. Han; C. Yang;S. K. Y. Tang

Comments

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Abbreviated Journal Title

Lab Chip

Keywords

IMAGE SENSORS; MICROFLUIDICS; DROPLETS; SINGLE; ARRAY; EMULSIONS; EVOLUTION; SYSTEMS; CHIP; Biochemical Research Methods; Chemistry, Multidisciplinary; Nanoscience; & Nanotechnology

Abstract

This paper describes an optofluidic droplet interrogation device capable of counting fluorescent drops at a throughput of 254000 drops per second. To our knowledge, this rate is the highest interrogation rate published thus far. Our device consists of 16 parallel microfluidic channels bonded directly to a filter-coated two-dimensional Complementary Metal-Oxide-Semiconductor (CMOS) sensor array. Fluorescence signals emitted from the drops are collected by the sensor that forms the bottom of the channel. The proximity of the drops to the sensor facilitates efficient collection of fluorescence emission from the drops, and overcomes the trade-off between light collection efficiency and field of view in conventional microscopy. The interrogation rate of our device is currently limited by the acquisition speed of CMOS sensor, and is expected to increase further as high-speed sensors become increasingly available.

Journal Title

Lab on a Chip

Volume

15

Issue/Number

6

Publication Date

1-1-2015

Document Type

Article

Language

English

First Page

1417

Last Page

1423

WOS Identifier

WOS:000350543500003

ISSN

1473-0197

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