Title

Morphology-Dependent HIV-Enhancing Effect of Semen-Derived Enhancer of Viral Infection

Authors

Authors

X. Qiao; J. Jeon; A. L. Cole; J. O. Matos; S. Bautista; J. Castillo; I. Hung; Z. H. Gan; S. A. Tatulian; A. M. Cole;B. Chen

Comments

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Abbreviated Journal Title

Biophys. J.

Keywords

BETA-AMYLOID FIBRILS; IMMUNODEFICIENCY-VIRUS TYPE-1; NUCLEAR-MAGNETIC-RESONANCE; THIOFLAVIN-T BINDING; ACID-PHOSPHATASE; MEDIATED ENHANCEMENT; CHEMICAL-SHIFT; CELL-FREE; SEVI; TRANSMISSION; Biophysics

Abstract

PAP248-286 is a 39-residue fragment (residues 248 to 286) derived from protease cleavage of prostatic acidic phosphatase in semen. The amyloid fibrils formed in vitro by PAP248-286 can dramatically enhance human immunodeficiency virus (HIV) infection. To our knowledge, we present the first report that the HIV-enhancing potency of fibrils formed by PAP248-286 is morphology dependent. We identified pleomorphic fibrils by transmission electron microscopy in two buffer conditions. Our solid-state NMR data showed that these fibrils consist of molecules in distinct conformations. In agreement with NMR, fluorescence measurements confirmed that they are assembled along different pathways, with distinct molecular structures. Furthermore, our cell-based infectivity tests detected distinct HIV-enhancing potencies for fibrils in distinct morphologies. In addition, our transmission electron microscopy and NMR results showed that semen-derived enhancer of viral infection fibrils formed in sodium bicarbonate buffer remain stable over time, but semen-derived enhancer of viral infection fibrils formed in phosphate buffered saline keep evolving after the initial 7 days incubation period. Given time, most of the assemblies in phosphate buffered saline will turn into elongated thin fibrils. They have similar secondary structure but different packing than thin fibrils formed initially after 7 days incubation.

Journal Title

Biophysical Journal

Volume

108

Issue/Number

8

Publication Date

1-1-2015

Document Type

Article

Language

English

First Page

2028

Last Page

2037

WOS Identifier

WOS:000353344400021

ISSN

0006-3495

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