New Two-Photon-Absorbing Probe with Efficient Superfluorescent Properties

    Authors

    K. D. Belfield; C. D. Andrade; C. O. Yanez; M. V. Bondar; F. E. Hernandez;O. V. Przhonska

    Comments

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    Abbreviated Journal Title

    J. Phys. Chem. B

    Keywords

    2-PHOTON ABSORPTION; ONE-PHOTON; FLUORENE DERIVATIVES; FLUORESCENCE; ANISOTROPY; MULTIPHOTON MICROSCOPY; CROSS-SECTIONS; EXCITATION; 3-PHOTON; NM; Chemistry, Physical

    Abstract

    The synthesis, linear photophysical, and photochemical parameters, two-photon absorption (2PA), and superfluorescence properties of 2,2'-(5,5'-(9,9-didecyl-9H-fluorene-2,7-diyl)bis(ethyne-2,1-diyl)bis(thiophene-5,2-diy1))dibenzo[d]thiazole (1) were investigated, suggesting its potential as an efficient fluorescent probe for bioimaging applications. The steady-state absorption, fluorescence, and excitation anisotropy spectra of 1 were measured in several organic solvents and aqueous media. Probe 1 exhibited high fluorescence quantum yield (similar to 0.7-0.8) and photochemical stability (photobleaching quantum yield similar to(3-7) x 10(-6)). The 2PA spectra were determined over a broad spectral range (640-920 nm) using a standard two-photon induced fluorescence method under femtosecond excitation. A well-defined two-photon allowed absorption band at 680-720 nm with corresponding 2PA cross sections delta(2PA) approximate to 800-900 GM was observed. The use of probe 1 in bioimaging was shown via one- and two-photon fluorescence imaging of HCT-116 cells. An amplification of the stimulated emission of 1 was demonstrated in organic solvents and thin polystyrene films, which potentially can be used for the development of new fluorescent labels with increased spectral brightness.

    Journal Title

    Journal of Physical Chemistry B

    Volume

    114

    Issue/Number

    44

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    14087

    Last Page

    14095

    WOS Identifier

    WOS:000283703400021

    ISSN

    1520-6106

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