Title

Comparison of the anaerobic microbiota of deep-water Geodia spp. and sandy sediments in the Straits of Florida

Authors

Authors

W. M. Bruck; T. B. Bruck; W. T. Self; J. K. Reed; S. S. Nitecki;P. J. McCarthy

Comments

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Abbreviated Journal Title

Isme J.

Keywords

anaerobic culture; FISH; Geodia spp.; marine sediment; TARGETED OLIGONUCLEOTIDE PROBES; SULFATE-REDUCING BACTERIA; IN-SITU; HYBRIDIZATION; CORAL-REEF SPONGES; IDENTIFICATION; POPULATIONS; COMMUNITY; SEA; DNA; MICROORGANISMS; Ecology; Microbiology

Abstract

Marine sediments and sponges may show steep variations in redox potential, providing niches for both aerobic and anaerobic microorganisms. Geodia spp. and sediment specimens from the Straits of Florida were fixed using paraformaldehyde and 95% ethanol (v/v) for fluorescence in situ hybridization (FISH). In addition, homogenates of sponge and sediment samples were incubated anaerobically on various cysteine supplemented agars. FISH analysis showed a prominent similarity of microbiota in sediments and Geodia spp. samples. Furthermore, the presence of sulfate-reducing and annamox bacteria as well as other obligate anaerobic microorganisms in both Geodia spp. and sediment samples were also confirmed. Anaerobic cultures obtained from the homogenates allowed the isolation of a variety of facultative anaerobes, primarily Bacillus spp. and Vibrio spp. Obligate anaerobes such as Desulfovibrio spp. and Clostridium spp. were also found. We also provide the first evidence for a culturable marine member of the Chloroflexi, which may enter into symbiotic relationships with deep-water sponges such as Geodia spp. Resuspended sediment particles, may provide a source of microorganisms able to associate or form a symbiotic relationship with sponges. The ISME Journal (2010) 4, 686-699; doi:10.1038/ismej.2009.149; published online 21 January 2010

Journal Title

Isme Journal

Volume

4

Issue/Number

5

Publication Date

1-1-2010

Document Type

Article

DOI Link

http://dx.doi.org/10.1038/ismej.2009.149

Language

English

First Page

686

Last Page

699

WOS Identifier

WOS:000277164000009

ISSN

1751-7362

Recommended Citation

"Comparison of the anaerobic microbiota of deep-water Geodia spp. and sandy sediments in the Straits of Florida" (2010). Faculty Bibliography 2010s. 7020.
https://stars.library.ucf.edu/facultybib2010/7020