Title

Filament-associated TSGA10 protein is expressed in professional antigen presenting cells and interacts with vimentin

Authors

Authors

A. Roghanian; D. C. Jones; J. V. Pattisapu; J. Wolfe; N. T. Young;B. Behnam

Comments

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Abbreviated Journal Title

Cell. Immunol.

Keywords

TSGA10; Vimentin; Dendritic cell; Macrophage; Intermediate filament; MICE LACKING VIMENTIN; TESTIS-SPECIFIC GENE; DENDRITIC CELLS; INTERMEDIATE-FILAMENTS; NEURONAL RECEPTOR; DIFFERENTIATION; IDENTIFICATION; ADHESION; ACTIN; TAIL; Cell Biology; Immunology

Abstract

Testis-specific gene antigen 10 (TSGA10) encodes an 82-kDa protein expressed during development, and in testis and brain tissues. We report its expression in human monocyte-derived dendritic cells (DC) and macrophages in vitro and in murine spleen CD11c(+) cells ex vivo. An interaction between DC/macrophage-derived TSGA10 and vimentin, as well as a few other major cytoskeletal proteins (e.g., actin-gamma 1), was identified by pull-down and mass spectroscopy assays. The interaction between TSGA10 and vimentin was further confirmed by immunoprecipitation and immunolocalisation in transfected RAW267 and HEK293 cell lines. TSGA10 formed filamentous structures in transfected COS-1 cells and was observed in cellular projections. We propose that TSGA10 could influence the function of antigen presenting cells (APC) via its interaction with cytoskeletal proteins such as vimentin. (C) 2010 Elsevier Inc. All rights reserved.

Journal Title

Cellular Immunology

Volume

265

Issue/Number

2

Publication Date

1-1-2010

Document Type

Article

Language

English

First Page

120

Last Page

126

WOS Identifier

WOS:000283472200006

ISSN

0008-8749

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