Identification of seroreactive proteins in the culture filtrate antigen of Mycobacterium avium ssp paratuberculosis human isolates to sera from Crohn's disease patients

    Authors

    A. R. Shin; H. J. Kim; S. N. Cho; M. T. Collins; E. J. B. Manning; S. A. Naser;S. J. Shin

    Comments

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    Abbreviated Journal Title

    FEMS Immunol. Med. Microbiol.

    Keywords

    Mycobacterium paratuberculosis; Crohn's disease; seroreactivity; diagnosis; FIBRONECTIN-ATTACHMENT PROTEIN; SUBSP PARATUBERCULOSIS; ANTIBODY-RESPONSE; HEAT-RESISTANCE; JOHNES-DISEASE; TUBERCULOSIS; PURIFICATION; COMPLEX; BOVIS; EXPRESSION; Immunology; Infectious Diseases; Microbiology

    Abstract

    The etiology of Crohn's disease (CD) is unresolved, but it is likely that an interplay of host genetic factors and environmental triggers is relevant. Mycobacterium paratuberculosis (MAP) has been focused upon as one of these triggers because it causes a similar chronic inflammatory bowel disease in animals. However, the differences among MAP antigens isolated from humans (H-MAP) and cattle (B-MAP) have not been well characterized. In this study, culture filtrate (CF) proteins from MAP isolates were tested with sera from CD patients and healthy controls in enzyme-linked immunosorbent assay (ELISA). Antibody produced by seven CD patients reacted differently according to the antigen source: strong reactivity was seen to H-MAP CF, but not to B-MAP CF. Six proteins, ModD, PepA, transaldolase, EchA9, MAP2120c, and MAP2950c, in H-MAP CF reacting specifically with CD patient sera were identified by liquid chromatography-electrospray ionization-MS. Bioinformatic analysis revealed that ModD and PepA were the same proteins reacting with sera from cattle infected with MAP. The elevated antibody responses of CD patients to rModD and rPepA were confirmed by ELISA (P < 0.001). These results support previous studies showing ModD and PepA as key antigens for the diagnosis of MAP infections. The study also identified additional proteins potentially useful in the design of assays for human MAP infections.

    Journal Title

    Fems Immunology and Medical Microbiology

    Volume

    58

    Issue/Number

    1

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    128

    Last Page

    137

    WOS Identifier

    WOS:000273549200014

    ISSN

    0928-8244

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