Unveiling the mechanism of uptake and sub-cellular distribution of cerium oxide nanoparticles

    Authors

    S. Singh; A. Kumar; A. Karakoti; S. Seal;W. T. Self

    Comments

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    Abbreviated Journal Title

    Mol. Biosyst.

    Keywords

    CARBON NANOTUBES; BIOMEDICAL APPLICATIONS; OXIDATIVE STRESS; MAMMALIAN-CELLS; CELLULAR UPTAKE; NANOCERIA; DRUG; DELIVERY; VACANCY; INTERNALIZATION; Biochemistry & Molecular Biology

    Abstract

    Cerium oxide nanoparticles (CNPs) have been recently studied for their potent superoxide scavenging properties in both cell and animal model systems. Data from these model systems have shown that exposure of cells to CNPs results in the protection against reactive oxygen species (ROS). Despite these exciting findings, very little is known regarding the uptake or subcellular distribution of these nanomaterials inside cells. In this study we utilized fluorophore (carboxyfluorescein) conjugated cerium oxide NPs (CCNPs) to study the mechanism of uptake and to elucidate the subcellular localization of CNPs using a keratinocyte model system. We observed rapid uptake (within 3 h) of CCNPs that was governed by energy-dependent, clathrin-mediated and caveolae-mediated endocytic pathways. We found CCNPs co-localized with mitochondria, lysosomes and endoplasmic reticulum as well as being abundant in the cytoplasm and the nucleus. Given the radical scavenging properties of cerium oxide and the widespread cellular disposition we observed, CNPs likely act as cellular antioxidants in multiple compartments of the cell imparting protection against a variety of oxidant injuries.

    Journal Title

    Molecular Biosystems

    Volume

    6

    Issue/Number

    10

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    1813

    Last Page

    1820

    WOS Identifier

    WOS:000281772300012

    ISSN

    1742-206X

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