Scanning Calorimetric Detections of Multiple DNA Biomarkers Contained in Complex Fluids

    Authors

    C. M. Wang; L. Y. Ma; L. M. Chen; K. X. Chai;M. Su

    Comments

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    Abbreviated Journal Title

    Anal. Chem.

    Keywords

    CIRCULATING TUMOR-CELLS; CANCER BIOMARKER; PROSTATE-CANCER; NANOPARTICLE; ELECTRODE; PROTEINS; SENSOR; Chemistry, Analytical

    Abstract

    Most of the existing techniques cannot be used to detect molecular biomarkers contained in complex fluids due to issues such as enzyme inhibition or signal interference. We have developed a nanoparticle-based scanning calorimetric method for the highly sensitive detections of multiple DNA biomarkers contained in cell lysate and milk by using solid-liquid phase change nanoparticles as thermal barcodes. The detection is based on the principle that the temperature of solid will not rise above die melting temperature unless all solid is molten, thus nanoparticles have sharp melting peaks during die thermal scan process. A one-to-one correspondence can thus be created between one type of nanoparticles and one type of biomarker, i.e., multiple biomarkers can be detected at the same time using it combination of nanoparticles. The melting temperature and the heat flow reflect the type and the concentration of the biomarker, respectively. The target oligonucleotides at low concentration in cell lysate (80 pM) have been detected through thermal signal transduction. The melting temperature of nanoparticles can be designed to avoid interference from coexisting species contained in the fluids, bringing simultaneously high sensitivity and multiplicity, as well as sample preparation benefits, to biomarker detections.

    Journal Title

    Analytical Chemistry

    Volume

    82

    Issue/Number

    5

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    1838

    Last Page

    1843

    WOS Identifier

    WOS:000274841300041

    ISSN

    0003-2700

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