Title

Scanning Calorimetric Detections of Multiple DNA Biomarkers Contained in Complex Fluids

Authors

Authors

C. M. Wang; L. Y. Ma; L. M. Chen; K. X. Chai;M. Su

Comments

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Abbreviated Journal Title

Anal. Chem.

Keywords

CIRCULATING TUMOR-CELLS; CANCER BIOMARKER; PROSTATE-CANCER; NANOPARTICLE; ELECTRODE; PROTEINS; SENSOR; Chemistry, Analytical

Abstract

Most of the existing techniques cannot be used to detect molecular biomarkers contained in complex fluids due to issues such as enzyme inhibition or signal interference. We have developed a nanoparticle-based scanning calorimetric method for the highly sensitive detections of multiple DNA biomarkers contained in cell lysate and milk by using solid-liquid phase change nanoparticles as thermal barcodes. The detection is based on the principle that the temperature of solid will not rise above die melting temperature unless all solid is molten, thus nanoparticles have sharp melting peaks during die thermal scan process. A one-to-one correspondence can thus be created between one type of nanoparticles and one type of biomarker, i.e., multiple biomarkers can be detected at the same time using it combination of nanoparticles. The melting temperature and the heat flow reflect the type and the concentration of the biomarker, respectively. The target oligonucleotides at low concentration in cell lysate (80 pM) have been detected through thermal signal transduction. The melting temperature of nanoparticles can be designed to avoid interference from coexisting species contained in the fluids, bringing simultaneously high sensitivity and multiplicity, as well as sample preparation benefits, to biomarker detections.

Journal Title

Analytical Chemistry

Volume

82

Issue/Number

5

Publication Date

1-1-2010

Document Type

Article

Language

English

First Page

1838

Last Page

1843

WOS Identifier

WOS:000274841300041

ISSN

0003-2700

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