Authors

X. H. Wang; S. Yao; H. Y. Ahn; Y. W. Zhang; M. V. Bondar; J. A. Torres;K. D. Belfield

Comments

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Abbreviated Journal Title

Biomed. Opt. Express

Keywords

Biochemical Research Methods; Optics; Radiology, Nuclear Medicine &; Medical Imaging

Abstract

Narrow dispersity organically modified silica nanoparticles (SiNPs), diameter similar to 30 nm, entrapping a hydrophobic two-photon absorbing fluorenyl dye, were synthesized by hydrolysis of triethoxyvinylsilane and (3-aminopropyl) triethoxysilane in the nonpolar core of Aerosol-OT micelles. The surface of the SiNPs were functionalized with folic acid, to specifically deliver the probe to folate receptor (FR) over-expressing Hela cells, making these folate two-photon dye-doped SiNPs potential candidates as probes for two-photon fluorescence microscopy (2PFM) bioimaging. In vitro studies using FR over-expressing Hela cells and low FR expressing MG63 cells demonstrated specific cellular uptake of the functionalized nanoparticles. One-photon fluorescence microscopy (1PFM) imaging, 2PFM imaging, and two-photon fluorescence lifetime microscopy (2P-FLIM) imaging of Hela cells incubated with folate-modified two-photon dye-doped SiNPs were demonstrated.

Journal Title

Biomedical Optics Express

Volume

1

Issue/Number

2

Publication Date

1-1-2010

Document Type

Article

Language

English

First Page

453

Last Page

462

WOS Identifier

WOS:000208209200012

ISSN

2156-7085

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