Abbreviated Journal Title
Biomed. Opt. Express
Keywords
Biochemical Research Methods; Optics; Radiology, Nuclear Medicine &; Medical Imaging
Abstract
Narrow dispersity organically modified silica nanoparticles (SiNPs), diameter similar to 30 nm, entrapping a hydrophobic two-photon absorbing fluorenyl dye, were synthesized by hydrolysis of triethoxyvinylsilane and (3-aminopropyl) triethoxysilane in the nonpolar core of Aerosol-OT micelles. The surface of the SiNPs were functionalized with folic acid, to specifically deliver the probe to folate receptor (FR) over-expressing Hela cells, making these folate two-photon dye-doped SiNPs potential candidates as probes for two-photon fluorescence microscopy (2PFM) bioimaging. In vitro studies using FR over-expressing Hela cells and low FR expressing MG63 cells demonstrated specific cellular uptake of the functionalized nanoparticles. One-photon fluorescence microscopy (1PFM) imaging, 2PFM imaging, and two-photon fluorescence lifetime microscopy (2P-FLIM) imaging of Hela cells incubated with folate-modified two-photon dye-doped SiNPs were demonstrated.
Journal Title
Biomedical Optics Express
Volume
1
Issue/Number
2
Publication Date
1-1-2010
Document Type
Article
Language
English
First Page
453
Last Page
462
WOS Identifier
ISSN
2156-7085
Recommended Citation
Wang, Xuhua; Yao, Sheng; Ahn, Hyo-Yang; Zhang, Yuanwei; Bondar, Mykhailo V.; Torres, Joseph A.; and Belfield, Kevin D., "Folate receptor targeting silica nanoparticle probe for two-photon fluorescence bioimaging" (2010). Faculty Bibliography 2010s. 923.
https://stars.library.ucf.edu/facultybib2010/923
Comments
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