Unveiling a Mystery: Investigating the Chlamydia ipaM Gene Using the Model Organism Drosophila

Authors

• Hillary Quach, University of Central FloridaFollow
• Vedwattie Ramdeo, University of Central FloridaFollow

Files

Course Code

MCB

Course Number

4920

Faculty/Instructor

Dr. George Aranjuez

Faculty/Instructor Email

George.Aranjuez@ucf.edu

About the Author

Hillary Quach is a junior and Vedwattie Ramdeo is a senior, both majoring in Biomedical Sciences. Many thanks to Dr. George Aranjuez for his mentorship and guidance in the GEAR program.

Abstract, Summary, or Creative Statement

In today's society, Chlamydia is becoming an increasingly serious concern. It is interesting to note that while the ipaM gene has never been studied in Drosophila, it is involved in Chlamydia’s life cycle. In this course, we are looking into different Chlamydia effector genes, specifically ipaM, to clone it into a transformation vector to produce transgenic Drosophila. With the collected DNA, a double digest was performed for the plasmid and PCR product. The ligated DNA will then be E. coli transformed and we intend to troubleshoot this. We are awaiting further data from observations of transgenic Drosophila with the cloned ipaM gene. Our expected results will bring light to ipaM’s function in relation to Chlamydia.

Keywords

Chlamydia; ipaM; Drosophila; DNA; Cloning; Transgenic; PCR; Transformation vector; Genetics

Recommended Citation

Quach, Hillary and Ramdeo, Vedwattie, "Unveiling a Mystery: Investigating the Chlamydia ipaM Gene Using the Model Organism Drosophila" (2023). High Impact Practices Student Showcase Fall 2023. 17.
https://stars.library.ucf.edu/hip-2023fall/17