High Impact Practices Student Showcase Fall 2023

Optimization of a Gel Electrophoresis Protocol to Enhance Results

Optimization of a Gel Electrophoresis Protocol to Enhance Results

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Download Sebastian Velez-PCB 4943-Research-Intensive-Amy Zeh Showcase- Poster.pptx (1.1 MB)

Course Code

PCB

Course Number

4943

Faculty/Instructor

Nicole Verity, Dr. Robert Borgon

Faculty/Instructor Email

Nicole.Verity@ucf.edu, Robert.Borgon@ucf.edu

Abstract, Summary, or Creative Statement

This experiment focused on an optimization protocol using DNA that students use in the laboratory component of the QBM teaching lab. Different agarose gel concentrations were tested, and 0.6% was found to create the best resolution and visibility for the given DNA. The experiment also helped me in becoming a better scientist as it taught me how to organize and structure myself in a research project, as well as how to be efficient with my time while producing good results.

Keywords

optimization protocol; agarose gel electrophoresis; peer instruction and laboratory training

Optimization of a Gel Electrophoresis Protocol to Enhance Results


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