Transfection of a neuronal cell line with the Wlds gene to further study its neuroprotective phenotype


Axon degeneration can result from primary damage due to a variety of causes, and in some instances, its effects can further propagate damage to vicinal neurons. When an axon has been damaged or transected, Wallerian degeneration is the apoptotic-like mechanism that is initiated, ultimately leading to the death of the neuron. The post-injury cellular inflammatory response is recruited to clear the degraded debris and is also responsible for activating the cascade events leading to additional cell death in surrounding neurons. A unique strain of mutant mice were discovered to express what is called the Wallerian Degeneration Slow (Wld') gene, which produces a chimeric nuclear protein that has been observed to dramatically delay both the onset of axon degeneration and the initiation of the post-injury cellular inflammatory response . Recent studies seem _to indicate the neuroprotective phenotype induced by the Wld' protein is the result of it modulating levels of genetic and protein expression in the damaged neuron. This thesis will review what is known about the Wld' protein and discuss how it offers an in-depth look at the molecular mechanisms behind neurodegenerative injury and disease states. In addition, we will discuss our efforts of isolating and purifying plasmids with the Wld' gene that have been cloned in order to create a stable cell line to aid in the future study for the characterization of neuroprotective mechanisms and the molecular pathways of neurodegeneration .


This item is only available in print in the UCF Libraries. If this is your thesis or dissertation, you can help us make it available online for use by researchers around the world by downloading and filling out the Internet Distribution Consent Agreement. You may also contact the project coordinator Kerri Bottorff for more information.

Thesis Completion





Sugaya, Kiminobu


Bachelor of Science (B.S.)


College of Medicine

Degree Program

Molecular Biology and Microbiology


Dissertations, Academic -- Medicine;Medicine -- Dissertations, Academic







Access Status

Open Access

Length of Campus-only Access


Document Type

Honors in the Major Thesis

This document is currently not available here.