Dab-1 over-expression increases acumination of Beta-catenin in HNSCs' nucleus and promotes differentiation in HNSC cells
The Reelin signaling pathway has been proven to play a critical role in human neural development, especially in the architectonic development of the central nervous system.
Extracelltilar Reeiin binds to the Very Low Density Lipoprotein Receptor (VLDLR) or the Apolipoprotein -E Receptor Type 2 (ApoER2) on the neural cell membrane and then induces tyrosine phosphorylation· o( the adapter protein Disabled - 1 (Dab-1 ). The phosphorylated Dab-I then cross talk with Wnt pathway to regulate gene expression. Recent researches have shown the Reelin pathway, or more specifically, Dab 1 over expression inhibits Glycogen Synthase Kinase 3P (GSK-3P) of the Wnt pathway. Taken the effect that inhibition of GSK-3P frees and promotes the P-Catenin acumination in cell cytosol and nucleus, and demonstrated by recent researches, increased level of neuron differentiation of GSK-3 p inhibited cell, we suggest that Dab-1 's ability of inhibit GSK- 3P will also result in increase level of P-Catenin in the human neural stem cells (HNSC), thus inducing the HNSC cells to differentiate. Testing the HNSC cells separately with Reelin conditioned media treatment and Dab-1 over-expression show significant increasing of acumination of P-Catenin in cell nucleus. Furthermore, demonstrated by our studies, Dab-1 over-expression also increases the neuron differentiation in HNSCs.
This item is only available in print in the UCF Libraries. If this is your thesis or dissertation, you can help us make it available online for use by researchers around the world by downloading and filling out the Internet Distribution Consent Agreement. You may also contact the project coordinator Kerri Bottorff for more information.
Bachelor of Science (B.S.)
College of Sciences
Dissertations, Academic -- Sciences;Sciences -- Dissertations, Academic
Length of Campus-only Access
Honors in the Major Thesis
Li, Meng, "Dab-1 over-expression increases acumination of Beta-catenin in HNSCs' nucleus and promotes differentiation in HNSC cells" (2009). HIM 1990-2015. 835.