Keywords
deoxyribozyme probe; single nucleotide substitution; NANOG; molecular diagnostics; split probes; glioblastoma
Abstract
The detection of single nucleotide variants in cancerous exosomes is emerging as a promising less-invasive diagnostics approach. In this study, a dual split deoxyribozyme probe (sDz) assay was designed to identify a single nucleotide substitution at position 442 in the NANOGP8 gene. One probe was designed to be highly specific to the 442-G target (S-G-2/U-4), and showed high selectivity and a high fluorescence signal for its specific target. The other probe (S-A+2/U-4) was designed to be non-selective and capable of binding to either target. This dual-probe system enabled a PCR-based fluorescence assay where the non-selective probe confirms the presence of the PCR amplified product, while the specific probe identifies the single nucleotide variant present. Trials with cancerous exosome samples showed that this dual-probe assay could effectively identify the presence of the A nucleotide substitution, associated with NANOGP8.
Thesis Completion Year
2025
Thesis Completion Semester
Spring
Thesis Chair
Gerasimova, Yulia
College
College of Sciences
Department
Chemistry
Thesis Discipline
Chemistry
Language
English
Access Status
Campus Access
Length of Campus Access
1 year
Campus Location
Orlando (Main) Campus
STARS Citation
Zavadskaya, Vera, "Development Of A Molecular Assay For The Single Nucleotide Substitution Detection At Position 442 In The Nanogp8 Gene" (2025). Honors Undergraduate Theses. 347.
https://stars.library.ucf.edu/hut2024/347
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