Abstract
The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting a antibiotic-free phytotoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation.
Document Type
Patent
Patent Number
US 7,795,497
Application Serial Number
10/741,379
Issue Date
9-14-2010
Current Assignee
Agency: University of Pennsylvania
Assignee at Issuance
Joint Assignment w/UCFRF: Auburn University
College
Burnett School of Biomedical Sciences
Department
Biomolecular Science Center
Allowance Date
4-15-2010
Filing Date
12-19-2003
Assignee at Filing
Joint Assignment w/UCFRF: Auburn University
Filing Type
Nonprovisional Application Record
Donated
no
Recommended Citation
Daniell, Henry, "Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection" (2010). UCF Patents. 312.
https://stars.library.ucf.edu/patents/312