Abstract
This invention relates to a body fluids identification method and kit. A parallel, multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay for the definitive identification of body fluids commonly encountered in forensic casework analysis, namely blood, saliva, semen, and vaginal secretions. The methodology is based on gene expression profiling analysis in which the body fluid-specific genes are identified by detecting the presence of appropriate messenger RNA species. Gene-specific primers are labeled with fluorescent dyes, separated and subjected to laser induced fluorescence for identification of body fluid-specific genes present in a sample stain.
Document Type
Patent
Patent Number
US 7,582,435
Application Serial Number
11/772,305
Issue Date
9-1-2009
Current Assignee
UCFRF
Assignee at Issuance
UCFRF
College
College of Sciences
Department
National Center for Forensic Science
Allowance Date
5-21-2009
Filing Date
7-2-2007
Assignee at Filing
UCFRF
Filing Type
Nonprovisional Application Record
Donated
no
Recommended Citation
Ballantyne, John and Juusola, Jane, "Messenger RNA Profiling: Body Fluid ID using Multiplex Reverse Transcription-polymerase chain reaction (RT-PCR) [DIV]" (2009). UCF Patents. 314.
https://stars.library.ucf.edu/patents/314