This invention relates to a body fluids identification method and kit. A parallel, multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay for the definitive identification of body fluids commonly encountered in forensic casework analysis, namely blood, saliva, semen, and vaginal secretions. The methodology is based on gene expression profiling analysis in which the body fluid-specific genes are identified by detecting the presence of appropriate messenger RNA species. Gene-specific primers are labeled with fluorescent dyes, separated and subjected to laser induced fluorescence for identification of body fluid-specific genes present in a sample stain.
Application Serial Number
Assignee at Issuance
College of Sciences
National Center for Forensic Science
Assignee at Filing
Nonprovisional Application Record
Ballantyne, John and Juusola, Jane, "Messenger RNA Profiling: Body Fluid ID using Multiplex Reverse Transcription-polymerase chain reaction (RT-PCR) [DIV]" (2009). UCF Patents. 314.