Abstract

A method of transforming plant plastids and regenerating fertile transplastomic plants by somatic embryogenesis is disclosed. The method involves transforming a plant plastid in a plant cell capable of being regenerated through somatic embryogenesis with a plastid expression cassette comprising one or more selectable marker genes that express in both green and non-green tissue and in light and dark conditions wherein the selectable marker plant cell is cultured in the presence of the plant cell to a selection agent. The transplastomic plant cell is cultured in the presence of the selection agent under conditions to cause the formation of a somatic embryo. The somatic embryo is grown into a fertile transplastomic plant. Preferably, the expression cassette contains two different selectable marker genes that express different proteins that provide plant cell resistance to the same selection agent.

Document Type

Patent

Patent Number

US 7,767,885

Application Serial Number

11/190,122

Issue Date

8-3-2010

Current Assignee

Agency: University of Pennsylvania

Assignee at Issuance

UCFRF

College

Burnett School of Biomedical Sciences

Department

Molecular Biology & Microbiology

Allowance Date

9-28-2009

Filing Date

7-25-2005

Assignee at Filing

UCFRF

Filing Type

Nonprovisional Application Record

Donated

no

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