Abstract

An ultra sensitive method for detection of biomolecules includes the step for providing a plurality of bioreceptor functionalized nanoparticle probes. The nanoparticles can include metal, semiconductor, radioactive isotope or fluorescent dye molecules. A sample solution suspected of including the target is contacted with the probes, wherein if present, the target binds to the bioreceptor. After such a binding a separating step follows. In the separating step, probes having the target bound thereto are separated from probes not having the target bound thereto. In one embodiment, probes having the target bound thereto are then decomposed to generate ions, or broken into discrete radioactive isotopes or fluorescent dye molecules to form a solution including a large plurality or metal ions, radioactive isotopes or dye molecules. A concentration of ions, radioactive isotopes or dye molecules in the solution is then determined and using this information the concentration of the target in the

Document Type

Patent

Patent Number

US 8,026,108 B1

Application Serial Number

11/875,252

Issue Date

9-27-2011

Current Assignee

UCFRF

Assignee at Issuance

UCFRF

College

NanoScience Technology Center

Department

NanoScience Technology Center

Allowance Date

5-27-2011

Filing Date

10-19-2007

Assignee at Filing

UCFRF

Filing Type

Nonprovisional Application Record

Donated

no

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