Abstract
Novel chimeric constructions and methods for their use are provided for expression of exogenous genes in a plant chloroplast. Particularly, expression is achieved by the use of a chloroplast or bacterial 5' untranslated region in the expression cassette. The expression cassette may be integrated into the chloroplast genome by the use of chloroplast DNA flanking sequences, or may replicate autonomously if provided with a chloroplast origin of replication. Plants and cells containing the transformed chloroplasts are also provided. The constructs may be used with both monocotyledenous and dicotyledenous chloroplasts.
Document Type
Patent
Patent Number
US 6,680,426 B2
Application Serial Number
10/223,160
Issue Date
1-20-2004
Current Assignee
Agency: Auburn University
Assignee at Issuance
Agency: Auburn University
College
Burnett School of Biomedical Sciences
Department
Molecular Biology & Microbiology
Allowance Date
3-7-2003
Filing Date
8-19-2002
Assignee at Filing
Agency: Auburn University
Filing Type
Nonprovisional Application Record
Donated
no
Recommended Citation
Daniell, Henry and McFadden, Bruce, "Genetic engineering of plant chloroplasts" (2004). UCF Patents. 704.
https://stars.library.ucf.edu/patents/704