Abstract

Some embodiments of the invention are directed to a microarray of binary nucleic acid probes for the detection of one or a plurality of nucleic acid analytes in a complex sample in a single high throughput assay with extraordinary specificity under physiologic conditions. Any binary nucleic acid probes that generate a detectable signal when bound to analyte are suitable for use in the microarrays, including binary deoxyribozyme or ribozyme probes; nonenzymatic binary probes for fluorescent detection, nonenzymatic binary dye-binding probes, and binary split enzyme peroxidase probes for visual detection of nucleic acids. The invention is also directed to new non nonenzymatic binary probes that bind to reporter oligonucleotides.

Document Type

Patent

Patent Number

US 8,853,134 B2

Application Serial Number

13/146,626

Issue Date

10-7-2014

Current Assignee

Agency: Columbia University

Assignee at Issuance

Agency: Columbia University

College

College of Sciences

Department

Chemistry

Allowance Date

2-12-2014

Filing Date

8-26-2011

Assignee at Filing

Agency: Columbia University

Filing Type

National Filing Record

Donated

no

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