Synthesis, liposome encapsulation, and evaluation of two-photon absorbing dyes for photodynamic cancer therapy

Keywords

Cancer -- Photochemotherapy, Fluorene, Liposomes

Abstract

The possibility ofusing two-photon absorbing compounds to treat cancer is very attractive for a number ofreasons. These reasons include the preferred use ofa nondamaging, near-IR light source, reduced light scattering, and a better depth ofpenetration with possible three-dimensional resolution. Although work has been done which demonstrates the possible utility oftwo-photon photodynamic therapy, the field is still in its infancy for two main reasons. The first is a general lack oftwo-photon absorbing materials for use in photodynamic therapy. The other is the organic nature ofthese dyes, which makes them difficult to get into the cell. The work outlined in this thesis is two-fold and designed to further the knowledge base in two-photon photodynamic therapy through investigations with new two-photon absorbing organic compounds. Several organic compounds were encapsulated in liposomes and introduced to tumor cells. The strate~y for encapsulating and evaluating these compounds was three fold. First we had to confirm encapsulation with fluorescence microscopy. The next 'Step was to verify cellular uptake and asses "dark" cytotoxicity. Finally, we needed to verify photodynamic therapeutic activity. Results of these three main experiments are presented, and provide the foundation for continued 11 research in this most promising field. The details surrounding the synthesis and evaluation ofa model compound are also presented. 111

Notes

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Graduation Date

2001

Advisor

Belfield, Kevin

Degree

Master of Science (M.S.)

College

College of Arts and Sciences

Department

Chemistry

Format

PDF

Pages

59 p.

Language

English

Length of Campus-only Access

None

Access Status

Masters Thesis (Open Access)

Identifier

DP0028712

Subjects

Arts and Sciences -- Dissertations, Academic; Dissertations, Academic -- Arts and Sciences

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