The effect of adherent cells, prostaglandin E2 (PGE2), and indomethacin on LAK cell activity of C57BL/6J (B6) mice was investigated. Depletion of adherent cells from splenocyte cultures prior to LAK cell generation increased LAK cell cytotoxicity by 60% compared to non-depleted splenocyte cultures. Depletion of adherent cells from splenocyte cultures following five days of incubation and prior to the LAK cell assay resulted in a similar enhancement. Indomethacin, an inhibitor of prostaglandin synthesis, enhanced LAK cell cytotoxicity in splenocyte cultures. PGE2 inhibited LAK cell cytotoxicity of adherent cell depleted splenocytes 28% and 88% at l0-6M and 10-5M. In vivo treatment of mice with indomethacin increased LAK cell cytotoxicity two fold compared to ethanol treated controls. LAK cell cytotoxicity was greatest in splenocytes from indomethacin treated mice cultured with additional indomethacin at 10.0 ug/ml. This study describes methods which increase murine LAK cell cytotoxicity.
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Longley, Ross E.
Master of Science (M.S.)
College of Arts and Sciences
Length of Campus-only Access
Masters Thesis (Open Access)
Roe, Kimberly G., "Inhibition of Murine Lymphokine Activated Killer (LAK) Cell Cytotoxicity by Adherent Cells and Prostaglandin E2" (1987). Retrospective Theses and Dissertations. 5050.