Title

Ultrastructural Imaging And Molecular Modeling Of Live Bacteria Using Soft X-Ray Contact Microscopy With Nanoseconds Laser-Plasma Radiation

Abstract

X-ray images of the various live bacteria, such as Staphylococcus and Streptococcus, and micromolecule such as chromosomal DNA from Escherichia coli, and Lipopolysaccharide from Burkholderia cepacia, are obtained with soft x-ray contact microscopy. A compact tabletop type glass laser system is used to produce x-rays from Al, Si, and Au targets. The PMMA photoresists are used to record x-ray images. An AFM (atomic force microscope) is used to reproduce the x-ray images from the developed photoresists. The performance of the 50nm spatial resolutions are achieved and images are able to be discussed on the biological view.

Publication Date

1-1-1995

Publication Title

Proceedings of SPIE - The International Society for Optical Engineering

Volume

2523

Number of Pages

194-201

Document Type

Article; Proceedings Paper

Personal Identifier

scopus

DOI Link

https://doi.org/10.1117/12.220979

Socpus ID

0029519923 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/0029519923

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