Title

Direct Ultrastructural Imaging Of Macrophages Using A Novel X-Ray Contact Microscopy

Abstract

Ultrastructural examination by electron microscopy (EM) involves a series of specialized preparation steps which may introduce artifacts in the micrographs. The development of high resolution laser-plasma x-ray contact microscopy is a new technique well suited to observe the intact structure of a biological specimen at high resolution without any specialized preparation steps. In the present study, we applied the x-ray microscopy technique to the study of macrophage ultrastructure as well as macrophage-microbes interaction. A compact table-top type Nd:glass laser system was used to produce x-rays. An atomic force microscope was used to obtain a digital image of the x-ray absorption profile from the developed polymethylmethacrylate photoresists which recorded the x ray images. BALB/c mouse thioglycolate-elicited peritoneal macrophages were cultured in RPMI 1640 medium with 10% fetal calf serum and infected with Candida albicans. By x-ray microscopy we have visualized the first x-ray images of macrophage ultrastructure as well as macrophage-C. albicans interactions. The success of x-ray images of macrophage ultrastructure presented reveals that x-ray contact microscopy is the great value in the study of the ultrastructure of live biological specimen.

Publication Date

12-1-1996

Publication Title

FASEB Journal

Volume

10

Issue

3

Number of Pages

-

Document Type

Article

Personal Identifier

scopus

Socpus ID

0343998227 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/0343998227

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