Title

Application of X-ray micrography and imaging to study the effect of gentamicin on Pseudomonas aeruginosa

Abstract

Aminoglycosides disrupt the outer membrane of Pseudomonas aeruginosa to facilitate access to their intracellular target. High-resolution X-ray micrography of live specimens is a relatively new technique. We used laser (nanosecond) plasma to image live cells of P. aeruginosa ATCC 27853. After exposure to 25 mg/L gentamicin for 15 min. we observed perturbation of the cell surface, membrane blebbings (370 nm and 273 nm diameter) away from the cell, formation of distinct channels (241 nm long) resulting from indentation and induction of cell elongation from 3-3.6 μm (control) to 4.6-5.26 μm (gentamicin-treated cells). These data illustrate the potential of high-resolution X-ray micrography for studying effects of drugs on live microbiological specimens.

Publication Date

5-1-1998

Publication Title

Journal of Antimicrobial Chemotherapy

Volume

41

Issue

5

Number of Pages

557-561

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1093/jac/41.5.557

Socpus ID

0031972258 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/0031972258

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