Title

Direct Ultrastructural Imaging Of Macrophages Using A Novel X-Ray Contact Microscopy

Abstract

A compact, high-resolution, laser-plasma, x-ray contact microscopy method using a table-top Nd:glass laser system has been developed. This x-ray microscopy system was applied for the observation of macrophage ultrastructures. These images were produced using proximity imaging in which a 5-ns pulse of soft x-rays with wavelengths near and inside the water windows (23å-44Å) produced by the laser-plasma were absorbed by the specimen and then registered on a photo resist. The x-ray images imprinted on the photo resist were then developed and analyzed with an atomic force microscope (AFM). Mouse thioglycollate-elicited peritoneal macrophages in suspension were examined by this new x-ray microscope. The x-ray images of the macrophages were compared with those observed by conventional transmission electron microscopy (TEM). The x-ray images showed no obvious organelles, including the nucleus and endoplasmic reticulum, as can be seen with TEM, but highland low-contrast structures caused by mass distribution of carbon were observed. Thus, using the x-ray microscopy we visualized the first x-ray images of macrophage ultrastructures. The successful x-ray imaging of macrophage ultrastructure indicates that proximity x-ray microscopy may be of value in studying physiology linked to the dynamics of a cell. Copyright © 1999 by the Society for Experimental Biology and Medicine.

Publication Date

1-1-1999

Publication Title

Experimental Biology and Medicine

Volume

220

Issue

1

Number of Pages

27-30

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1046/j.1525-1373.1999.d01-4.x

Socpus ID

0032897224 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/0032897224

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