Title

Immunodetection And Comparison Of Melaleuca, Bottlebrush, And Bahia Pollens

Keywords

Allergen characterization; Allergenic cross-reactivity; Callistemon citrinis; Immunoblotting; Melaleuca leucadendron; Paspalum notation

Abstract

Pollen extracts of two trees, Callistemon citrinis (bottlebrush) and Melaleuca leucadendron (melaleuca), as well as the grass Paspalum notation (bahia) were analyzed for antigenic and allergenic cross-reactivity using SDS-PAGE and Western blotting. SDS-PAGE analysis of all three pollen extracts revealed multiple antigenic components which were reactive with rabbit antisera made to each pollen extract. Comparison of reduced and nonreduced mobility patterns suggested the possible presence of multichain proteins linked by disulfide bonds. Clinical studies demonstrated that 81% of the patients skin test positive to at least one of the pollens were also positive to the other two. Sixty-three percent of allergic individuals studied showed a high correlation between skin test results and the number of IgE-binding components analyzed by immunoblotting. These IgE-reactive components were detected in the molecular weight range of 29- 66 kD. Western blot analysis detected more IgE-binding components in bahia pollen extracts as compared with the tree pollens, consistent with other reports of grass pollens being more allergenic than those from trees and weeds. Each patient’s serum had a unique IgE-binding pattern, indicating heterogeneity of immune response; however, common major determinants were detected by a large percentage of the allergic patient’s sera. © 1994 S. Karger AG, Basel.

Publication Date

1-1-1994

Publication Title

International Archives of Allergy and Immunology

Volume

105

Issue

3

Number of Pages

289-296

Document Type

Article

Identifier

scopus

Personal Identifier

scopus

DOI Link

https://doi.org/10.1159/000236771

Socpus ID

0028032718 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/0028032718

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