Title

Expression Of Green-Fluorescent Protein Gene In Sweet Potato Tissues

Keywords

Electroporation; Green-fluorescent protein; Intact cells; Ipomoea batatas; Particle bombardment; Protoplasts; Stable expression; Sweet potato

Abstract

Green-fluorescent protein (GFP) gene expression, transient and stable after electroporation and particle bombardment, was analyzed in tissues of sweet potato cv. Beauregard. Leaf and petiole tissues were used for protoplast isolation and electroporation. After 48 h, approximately 25-30% of electroporated mesophyll cell protoplasts regenerated cell walls, and of these, 3% expressed GFP. Stable expression of GFP after four weeks of culture was observed in 1.0% of the initial GFP positive cells. In a separate experiment, we observed 600-700 loci expressing GFP 48 h after bombarding leaf tissue or embryogenic calli, and stable GFP-expressing sectors were seen in leaf-derived embryogenic calli after four weeks of protoplast culture without selection. These results demonstrate GFP gene expression in sweet potato tissues. Screening for GFP gene expression may prove useful to improve transformation efficiency and to facilitate detection of transformed sweet potato plants.

Publication Date

1-1-2000

Publication Title

Plant Molecular Biology Reporter

Volume

18

Issue

2

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1007/BF02824022

Socpus ID

0442326197 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/0442326197

This document is currently not available here.

Share

COinS