Title

A One-Step Highly Sensitive Method For Dna Detection Using Dynamic Light Scattering

Abstract

A one-step homogeneous DNA detection method with high sensitivity was developed using gold nanoparticles (AuNPs) coupled with dynamic light scattering (DLS) measurement. Citrate-protected AuNPs with a diameter of 30 nm were first functionalized with two sets of single-stranded DNA probes and then used as optical probes for DNA detection. In the presence of target DNA, the hybridization between target DNA and the two nanoparticle probes caused the formation of nanoparticle dimers, trimers, and oligomers. As a result, the nanoparticle aggregation increased the average diameter of the whole nanoparticle population, which can be monitored simply by DLS measurement. A quantitative correlation can be established between the average diameter of the nanoparticles and the target DNA concentration. This DLS-based assay is extremely easy to conduct and requires no additional separation and amplification steps. The detection limit is around 1 pM, which is 4 orders of magnitude better than that of light-absorption-based methods. Single base pair mismatched DNAs can be readily discriminated from perfectly matched target DNAs using this assay. Copyright © 2008 American Chemical Society.

Publication Date

7-2-2008

Publication Title

Journal of the American Chemical Society

Volume

130

Issue

26

Number of Pages

8138-8139

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/ja801947e

Socpus ID

46049095695 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/46049095695

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