Title
Temporal Neurotransmitter Conditioning Restores The Functional Activity Of Adult Spinal Cord Neurons In Long-Term Culture
Keywords
Acetylcholine-chloride; Adult rat spinal cord neurons; Electrophysiology; Glutamate (N-acetyl-dl-glutamic acid); Motoneuron; Neurotransmitters; Regeneration; Serotonin; Serum-free medium; Silane substrate
Abstract
The ability to culture functional adult mammalian spinal cord neurons represents an important step in the understanding and treatment of a spectrum of neurological disorders including spinal cord injury. Previously, the limited functional recovery of these cells, as characterized by a diminished ability to initiate action potentials and to exhibit repetitive firing patterns, has arisen as a major impediment to their physiological relevance. In this report, we demonstrate that single temporal doses of the neurotransmitters serotonin, glutamate (N-acetyl-dl-glutamic acid) and acetylcholine-chloride lead to the full electrophysiological functional recovery of adult mammalian spinal cord neurons, when they are cultured under defined serum-free conditions. Approximately 60% of the neurons treated regained their electrophysiological signature, often firing single, double and, most importantly, multiple action potentials. © 2007 Elsevier Inc. All rights reserved.
Publication Date
1-1-2008
Publication Title
Experimental Neurology
Volume
209
Issue
1
Number of Pages
171-180
Document Type
Article
Personal Identifier
scopus
DOI Link
https://doi.org/10.1016/j.expneurol.2007.09.019
Copyright Status
Unknown
Socpus ID
37549055489 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/37549055489
STARS Citation
Das, Mainak; Bhargava, Neelima; Bhalkikar, Abhijeet; Kang, Jung Fong; and Hickman, James J., "Temporal Neurotransmitter Conditioning Restores The Functional Activity Of Adult Spinal Cord Neurons In Long-Term Culture" (2008). Scopus Export 2000s. 10412.
https://stars.library.ucf.edu/scopus2000/10412