Title

Temporal Neurotransmitter Conditioning Restores The Functional Activity Of Adult Spinal Cord Neurons In Long-Term Culture

Keywords

Acetylcholine-chloride; Adult rat spinal cord neurons; Electrophysiology; Glutamate (N-acetyl-dl-glutamic acid); Motoneuron; Neurotransmitters; Regeneration; Serotonin; Serum-free medium; Silane substrate

Abstract

The ability to culture functional adult mammalian spinal cord neurons represents an important step in the understanding and treatment of a spectrum of neurological disorders including spinal cord injury. Previously, the limited functional recovery of these cells, as characterized by a diminished ability to initiate action potentials and to exhibit repetitive firing patterns, has arisen as a major impediment to their physiological relevance. In this report, we demonstrate that single temporal doses of the neurotransmitters serotonin, glutamate (N-acetyl-dl-glutamic acid) and acetylcholine-chloride lead to the full electrophysiological functional recovery of adult mammalian spinal cord neurons, when they are cultured under defined serum-free conditions. Approximately 60% of the neurons treated regained their electrophysiological signature, often firing single, double and, most importantly, multiple action potentials. © 2007 Elsevier Inc. All rights reserved.

Publication Date

1-1-2008

Publication Title

Experimental Neurology

Volume

209

Issue

1

Number of Pages

171-180

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.expneurol.2007.09.019

Socpus ID

37549055489 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/37549055489

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