Title

Regeneration Of Adult Mice Motoneurons Utilizing A Defined System And Anti-Oxidant Nanoparticles

Keywords

Adult mice; Adult motoneuron; Antioxidant; Cerium oxide nanoparticles; Serum-free medium; Silane substrate; Spinal cord

Abstract

We have developed a novel culture model to grow adult mice spinal cord cells. The model consisted of a serum-free medium supplemented with different growth factors, nanoparticles and a silane substrate. The following growth factors and nanoparticles constitute the novel serum-free medium: Acidic FGF, Heparan-Sulphate, GDNF, BDNF, CNTF, CT-1, NT-3, NT-4, VEGF, LIF, Vitronectin, B27 supplement, G5 supplement and Cerium oxide nanoparticles. Synergistic effects of these growth factors, nanoparticles and silane substrate promote survival and growth of spinal-cord neurons and glial cells. Based on the cell body size, number of processes and process lengths the cells were categorized into three different groups: Groups I, II and III. Apart from these three cell groups, some of the cells grew in clumps. We categorized the clumps as Group IV. Cells were immunocytochemically characterized by antibodies against NF-150 and GFAP. The cells in Group I, which were found in a small percentage (∼10%) in each culture, were then further characterized. These cells had large, multi-polar, cell bodies (30-35 μm) and long processes (∼500-800 μm) and they also stained positive for all three motoneuron specific antibodies; ChAT, Islet-1 and MO-1. Based on morphological and immunocytochemical analysis it was concluded that this small percentage of large, multi-polar cells were regenerating motoneurons. Preliminary electrophysiological studies indicated that the cells with this neuronal morphology fired single action potentials. This novel model system could be used as a tool to study spinal injuries and neurodegenerative diseases of aging spinal cord. Copyright © 2009 American Scientific Publishers. All rights reserved.

Publication Date

12-1-2009

Publication Title

Journal of Nanoneuroscience

Volume

1

Issue

2

Number of Pages

130-143

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1166/jns.2009.1002

Socpus ID

77953309060 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/77953309060

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