Novel Murine Model For Colon Cancer: Non-Operative Trans-Anal Rectal Injection


colon cancer; non-operative injection of cancer cells.; orthotopic murine model


Background: This study was conducted to develop a modified murine model of colon cancer that is non-operative. Currently, the most accurate orthotopic murine model of colon cancer requires an invasive procedure involving cecal injection of colon cancer cells and therefore limits the ability to perform immunological studies subsequent to cecal resections. Materials and methods: Murine colon cancer (CT26) cells were injected submucosally into the distal, posterior rectum of BALB/c mice. Care was taken not to pass transmurally into the pelvic cavity. Different magnifications (10× versus 100×) were used for injection, and primary tumor growth and metastatic disease were studied. Results: In the initial study, 3/7 mice injected using 10× magnifications had notable, large tumor originating from the rectal wall, and histology revealed that all excised tumors were poorly differentiated adenocarcinoma. In the second study, 8/10 mice injected using 100× magnifications had notable tumor originating from the rectal well, and 4/8 mice had abnormal lung tissue with pathological evidence of hemorrhagic pulmonary edema. The use of 10× magnification resulted in 43% tumor take. In sharp contrast, 80% tumor take was observed with 100× magnification. The overall success of tumor take was 65% using the trans-anal rectal injection model. Conclusions: Our modified orthotopic murine model of colon cancer offers an alternative non-operative murine model for colon cancer and is less invasive than the traditional orthotopic model (i.e., cecal injection). This model may allow for more accurate investigations of inflammation and immune responses to surgical intervention without the influence of previous abdominal surgery. © 2009 Elsevier Inc. All rights reserved.

Publication Date


Publication Title

Journal of Surgical Research





Number of Pages


Document Type


Personal Identifier


DOI Link


Socpus ID

67349161634 (Scopus)

Source API URL


This document is currently not available here.