Title

Tetranucleotide Markers From The Loggerhead Sea Turtle (Caretta Caretta) And Their Cross-Amplification In Other Marine Turtle Species

Keywords

Caretta caretta; Cross-amplification; Loggerhead turtle; Microsatellites

Abstract

The loggerhead sea turtle (Caretta caretta) is a federally threatened species and listed as endangered by the World Conservation Union (IUCN). We describe primers and polymerase chain reaction (PCR) conditions to amplify 11 novel tetranucleotide microsatellite loci from the loggerhead sea turtle. We tested primers using samples from 22 females that nested at Melbourne Beach, Florida (USA). Primer pairs yielded an average of 11.2 alleles per locus (range of 4-24), an average observed heterozygosity of 0.83 (range 0.59-0.96), and an average polymorphic information content of 0.80 (range 0.62-0.94). We also demonstrate the utility of these primers, in addition to primers for 15 loci previously described, for amplifying microsatellite loci in four additional species representing the two extant marine turtle families: olive ridley (Lepidochelys olivacea), hawksbill (Eretmochelys imbricata), green turtle (Chelonia mydas), and leatherback (Dermochelys coriacea). © 2008 Springer Science+Business Media B.V.

Publication Date

6-1-2009

Publication Title

Conservation Genetics

Volume

10

Issue

3

Number of Pages

577-580

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1007/s10592-008-9573-6

Socpus ID

67349263061 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/67349263061

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