Title

Acetylation Of Fluoroquinolone Antimicrobial Agents By An Escherichia Coli Strain Isolated From A Municipal Wastewater Treatment Plant

Keywords

Acetylation; Antimicrobial agents; Escherichia coli; Fluoroquinolones; Quinolone-resistance determining regions; Resistance; Transformation; Wastewater

Abstract

Aims: To isolate environmental bacteria capable of transforming fluoroquinolones to inactive molecules. Methods and Results: Bacteria were isolated from the aerobic liquor of a wastewater treatment plant on a medium containing norfloxacin (100 mg l-1). Twenty-two isolates were highly resistant (minimal inhibitory concentration: 6.25-200 μg ml-1) to five fluoroquinolones and six of them were positive by PCR amplification for the aminoglycoside resistance gene aac(6′)-Ib. Of these, only Escherichia coli strain LR09 had the ciprofloxacin-acetylating variant gene aac(6′)-Ib-cr; HPLC and mass spectrometry showed that this strain transformed both ciprofloxacin and norfloxacin by N-acetylation. This bacterium also had mutations in the quinolone-resistance determining regions of the gyrA and parC genes. Conclusions: An E. coli isolate from wastewater, which possessed at least two distinct fluoroquinolone resistance mechanisms, inactivated ciprofloxacin and norfloxacin by N-acetylation. Significance and Impact of the Study: This is the first report of N-acetylation of fluoroquinolones by an aac(6′)-Ib-cr-containing bacterium from an environmental source. © 2009 The Authors.

Publication Date

2-1-2009

Publication Title

Journal of Applied Microbiology

Volume

106

Issue

2

Number of Pages

564-571

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1111/j.1365-2672.2008.04026.x

Socpus ID

58549086167 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/58549086167

This document is currently not available here.

Share

COinS